Imaging morphological details and pathological differences of red blood cells using tapping-mode AFM

The surface topography of red blood cells (RBCs) was investigated under nearphysiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly tethered to the support. This results in a tight but noninvasive attachment of the cells. Using tappingmode AFM, which is known as gentle imaging mode and therefore most appropriate for soft biological samples like erythrocytes, it was possible to resolve membrane skeleton structures without major distortions or deformations of the cell surface. Significant differences in the morphology of RBCs from healthy humans and patients with systemic lupus erythematosus (SLE) were observed on topographical images. The surface of RBCs from SLE patients showed characteristic circularshaped holes with approx. 200 nm in diameter under physiological conditions, a possible morphological correlate to previously published changes in the SLE erythrocyte membrane

Dynamic force microscopy for imaging of viruses under physiological conditions

Dynamic force microscopy (DFM) allows imaging of the structure and the assessment of the function of biological specimens in their physiological environment. In DFM, the cantilever is oscillated at a given frequency and touches the sample only at the end of its downward movement. Accordingly, the problem of lateral forces displacing or even destroying bio-molecules is virtually inexistent as the contact time and friction forces are reduced. Here, we describe the use of DFM in studies of human rhinovirus serotype 2 (HRV2) weakly adhering to mica surfaces. The capsid of HRV2 was reproducibly imaged without any displacement of the virus. Release of the genomic RNA from the virions was initiated by exposure to low pH buffer and snapshots of the extrusion process were obtained. In the following, the technical details of previous DFM investigations of HRV2 are summarized

Twenty-first century glacier slowdown driven by mass loss in High Mountain Asia

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Atomic Force Microscopy Images of Various Specimens

This data set consists of ten atomic force microscopy images in MI format as well as corresponding previews in PNG format. The microscopy images are of various materials and have been scanned with AFM equipment from Keysight Technologies. Details on the individual images: image_7.mi - calibration grid with 5 µm pitch size image_8.mi - Celgard (a polymer membrane used in batteries) image_9.mi - Titanium-Tungsten film image_10.mi - AFM image image_11.mi - self-assembled monolayer of lipids on gold image_12.mi - capacity calibration sample for Scanning Microwave Microscopy imaging image_13.mi - capacity calibration sample for Scanning Microwave Microscopy imaging image_14.mi - PS-LDPE-12M (a polymer blend of Polystyrene and Polyolefin Elastomere) image_15.mi - AFM Calibration grid image_16.mi - AFM Calibration grid The images can be opened using, e.g. Gwyddion: http://gwyddion.net/ The Python package Magni can be used to load the images into Python (using the magni.afm.io module): https://github.com/SIP-AAU/Magni The images are provided as-is without warranty of any kind

Glacier changes in the Karakoram region mapped by multimission satellite imagery, links to GeoTIFF and ESRI shape file

Positive glacier-mass balances in the Karakoram region during the last decade have fostered stable and advancing glacier termini positions, while glaciers in the adjacent mountain ranges have been affected by glacier recession and thinning. In addition to fluctuations induced solely by climate, the Karakoram is known for a large number of surge-type glaciers. The present study provides an updated and extended inventory on advancing, stable, retreating, and surge-type glaciers using Landsat imagery from 1976 to 2012. Out of 1219 glaciers the vast majority showed a stable terminus (969) during the observation period. Sixty-five glaciers advanced, 93 glaciers retreated, and 101 surge-type glaciers were identified, of which 10 are new observations. The dimensional and topographic characteristics of each glacier class were calculated and analyzed. Ninety percent of nonsurge-type glaciers are shorter than 10 km, whereas surge-type glaciers are, in general, longer. We report short response times of glaciers in the Karakoram and suggest a shift from negative to balanced/positive mass budgets in the 1980s or 1990s. Additionally, we present glacier surface velocities derived from different SAR (synthetic aperture radar) sensors and different years for a Karakoram-wide coverage. High-resolution SAR data enables the investigation of small and relatively fast-flowing glaciers (e.g., up to 1.8 m/day during an active phase of a surge). The combination of multitemporal optical imagery and SAR-based surface velocities enables an improved, Karakoram-wide glacier inventory and hence, provides relevant new observational information on the current state of glaciers in the Karakoram

Spectral-Coding-Based Compressive Single-Pixel NIR Spectroscopy in the Sub-Millisecond Regime

In this contribution, we present a high-speed, multiplex, grating spectrometer based on a spectral coding approach that is founded on principles of compressive sensing. The spectrometer employs a single-pixel InGaAs detector to measure the signals encoded by an amplitude spatial light modulator (digital micromirror device, DMD). This approach leads to a speed advantage and multiplex sensitivity advantage atypical for standard dispersive systems. Exploiting the 18.2 kHz pattern rate of the DMD, we demonstrated 4.2 ms acquisition times for full spectra with a bandwidth of 450 nm (5250–4300 cm−1; 1.9–2.33 µm). Due to the programmability of the DMD, spectral regions of interest can be chosen freely, thus reducing acquisition times further, down to the sub-millisecond regime. The adjustable resolving power of the system accessed by means of computer simulations is discussed, quantified for different measurement modes, and verified by comparison with a state-of-the-art Fourier-transform infrared spectrometer. We show measurements of characteristic polymer absorption bands in different operation regimes of the spectrometer. The theoretical multiplex advantage of 8 was experimentally verified by comparison of the noise behavior of the spectral coding approach and a standard line-scan approach

Terahertz Time-Domain Polarimetry in Reflection for Film Characterization

Terahertz time-domain spectroscopy is a useful technique to characterize layered samples and thin films. It gives access to their optical properties and thickness. Such measurements are done in transmission, which requires access to the sample from opposite sides. In reality this is not always possible. In such cases, reflection measurements are the only option, but they are more difficult to implement. Here we propose a method to characterize films in reflection geometry using a polarimetric approach based on the identification of Brewster angle and modeling of the measured signal to extract the refractive index and thickness of the sample. The technique is demonstrated experimentally on an unsupported single layer thin film sample. The extracted optical properties and thickness were in good agreement with established transmission terahertz spectroscopy measurements. The new method has the potential to cover a wide range of applications, both for research and industrial purposes